ܹü/  Proteomics Core Facility
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02-880-4434 / 504 506ȣ
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м (mass spectrometry, MS) ߽ ̷ ܹ ϰų ƴ϶ Ȯ ִ ſ ߿ мԴϴ. ܹ м ܹ in-gel Ȥ in-solution ¿ Ʈ ȿҷ Ͽ Ÿ̵ , Ÿ̵带 üũθ׷Ƿ и/Ͽ м Ӱ ÿ мon-line ϴ LC-MS based proteomics մϴ. м Ÿ̵ (precursor ion) ڷ Ŀ, Ÿ̵带 ü󿡼 (daughter ions) ڷ մϴ. ̷ ν Ÿ̵ ڷ ܹ ÷ᰡ genome sequence ź Ÿ̵带 ãƳ ܹ ϰ ˴ϴ

Ʒ workflow Ǿ , ̷ LC-MS ܹü , имȭ, м, ϳ ̷ йԴϴ.    

 

Workflow of proteomics research   







мý м մϴ. к Thermo LTQ MS coomassie-stainable gel ÷ῡ,  IBS RNA Thermo Q-Exactive MS silver-stained gel ѵ ÷ ܹ м մϴ. ̵ м in-letġ üũθ׷ ü ۵ ÷ 𼼰 ÷ ׷ ϴ Դϴ 


к ٸ ܹ м մϴ. ܺοҿ ü , к ʴ ֽϴ. 

ܹ м Ƿڴ ֽñ ٶϴ (02-880-4434).

 

 

1. Thermo LTQ MS
 

2005 к Եion trap źмԴϴ. Coomssie-stainable gel band ܹ м Ȱ˴ϴ. ̿ȭġ Ͽ, üũθ׷ǿ in-house packed 𼼰 ÷ Ͽ ׽ϴ.

 

2.   Thermo Q-Exactive MS
 

2013 IBS RNA Ե , quadrupole mass filter orbitrap źмԴϴ. Orbitrap Ʈ شɰ Ȯ ſ ƴ϶, 12 Hz HCD MS/MS Ͽ ܹü о߿ м⺸ մϴ. ܼ ܹ DZ ٴ systems biology Ȥ system-wide target discovery , ׸ SILAC quantitative proteomics ˴ϴ.

 

3.  Agilent 6490 MS


2013 IBS RNA Ե , triple quadrupole (QQQ) źмԴϴ. м ܹ м, ܹ м η , ܹ м Ưȭ μ ϰ ֽϴ. ̷ Ͽ Ϲ мδ ϴ ܹ Ʒ , Western blot assay ܹ մϴ.

 

4. Waters nanoAcquity

  

2013 IBS RNA Ե , м in-let ܹ Ÿ̵带 ش и/Ͽ м ̴ մϴ. 10,000 psi (690 bar) ̵ nanoflow ( nL/mn) оָ, и ſ پ Դϴ.

 

5. Thermo Ultimate RSLCnano

   

2014 IBS RNA Ե , м in-let Ǹ ܹ Ÿ̵带 ش и/Ͽ м ̴ մϴ. 14,500 psi (1,000 bar) ʰ ̵ nanoflow ( nL/mn) о ־, sub 2 µm ۵ 50 cm ̻ ʰ 𼼰 ÷  ϴµ ˴ϴ.

 

6. Agilent 1290 UPLC

 

18,000 psi (1,240 bar) ʰ ̵ capillary flow micro flow , DAD UV fraction collector Ͽ ش Ÿ̵带 fractionationϴµ ַ Ȱ˴ϴ. Agilent 6490 MS in-let DZ⵵ մϴ.

7 Customized capillary column packing system

 

20,000 psi ʰ ϴ ISCO syringe pump ü 𼼰 ÷ ý Ͽϴ. ʰ 𼼰 ÷ ü Ͽ м Ȱϰ ֽϴ.

ڿ Ϻ